«AVIVAC-REO» strain 1133

About the product

Live dry vaccine against avian reovirus tenosynovitis based on virus strain 1133 with supplied diluent.

The vaccine is prepared from attenuated avian reovirus strain 1133 propagated in fibroblast cells from SPF chicken embryos in the presence of a stabilizing agent. The vaccine has the appearance of a homogenous yellowish brown or pink porous substance that easily dissolves in water or normal saline without forming flakes or sediment. The vaccine is available in hermetically sealed vials containing 1 to 4 cm³ of the product, corresponding to 50–3000 doses per vial. The diluent supplied with the vaccine is available in 200 or 400 cm³ glass vials.

Biological properties

One immunizing dose of the vaccine contains not less than 10⁴ TCID₅₀/cm³ avian reovirus. Immunity in vaccinated chickens forms over a period of 3 weeks after double vaccination and lasts 10 to 12 weeks. To induce lifelong immunity it is advisable to revaccinate the birds at the age of 100–110 days with AVIVAC’s inactivated vaccine against avian reovirus tenosynovitis in accordance with the Instructions for use.

The vaccine is innocuous and non-reactogenic; it possesses no therapeutic properties.

Indications for use

The vaccine is used for prophylactic immunization of birds at farm units affected by reovirus tenosynovitis or in the presence of a risk thereof. Only clinically healthy birds should undergo vaccination. Birds should be vaccinated twice, at the age of 7 to 10 days and at the age of 35 to 40 days.

The vaccine is administered subcutaneously at a rate of 10⁴ TCID_50/cm³ per 0.2cm³ diluent. The injection site should be disinfected with 70% ethanol. The vial containing the diluted vaccine should be shaken intermittently. Robustness of immunity is tested 21 days post vaccination. Blood serum samples from 20 to 25 birds are tested by performing an ELISA assay following the conventional procedure.

Vaccination is considered successful if not less than 80% of the immunized chickens demonstrate mean antibody titres against avian reovirus exceeding the minimum threshold value specified by the manufacturer of the kit by 100% or more.

Storage requirements

The shelf life of the live dry vaccine is 12 months from the date of manufacture if stored and transported in a dark dry place at a temperature between +2 and +8°С.
The shelf life of the diluent is 24 months if stored and transported in a dry dark place at a temperature between +2 and +15°С.